This repository contains scripts for the 'Spatiotemporally resolved single-cell transcriptomics reveals kinetic sculpting of RNA life cycle'.
Image processing(Image registration, spot calling, and barcode filtering)Cell processing(RNA cytoplasmic translocation modeling and estimation in HeLa cells)Kinetic analysis(RNA kinetic parameter modeling and estimation (including synthesis, degradation, nuclear export) in HeLa cells，human induced pluripotent stem cell-derived cariomyocytes (hiPSC-CMs) and primary human skin cells)Other cell lines(other data processing and RNA cytoplasmic translocation modeling and estimation in hiPSC-CM and skin cell dataset)
The related datasets can be found:
- Please use the most recent version of the dataset.
If you find TEMPOmap useful for your work, please cite our paper: https://www.nature.com/articles/s41592-023-01829-8.
Ren, J., Zhou, H., Zeng, H. et al. Spatiotemporally resolved transcriptomics reveals the subcellular RNA kinetic landscape. Nat Methods (2023). https://doi.org/10.1038/s41592-023-01829-8
Contact us at [email protected] if you have any issues.
Jiahao Huang, Haowen Zhou, Connie Wang, Jingyi (Rena) Ren