This repository offer a complementory R script to a scientific paper which will soon be published (currently pending review - reference will be added accordingly). The goal of this script is to facilitate the calculation of binding parameters (and corresponding errors) associated with Langmuir monolayers experiments.
You can either clone the git repository locally or simply download the R script file named binding-parameters-calculations.R.
Then, using your preferred R interpreter (eg RStudio; a more exhaustive list can be found here), open the R script.
We provide you with an example data set (example-data.csv) which can also be downloaded and used as trial data. The first column has to be Πi data and the second column ΔΠ data.
Your data has to be provided as a csv file where the first line corresponds to headers. Alternatively, you may modify the R script to suit your data format.
The script will read your data and generate a ΔΠ vs Πi plot like the following:
The authors do not pretend to great artistic talent. You are therefore encouraged to modify the resulting plot to your needs and artistic capabilities. R offers various packages for plot generation.
References for the calculations:
- Calvez et al. (2009) Biochimie 91:718. (Pubmed)
- Calvez et al. (2011) Langmuir 27:1373. (Pubmed)
- Boisselier et al. (2017) Advances in Colloid and Interface Science 243:60. (Pubmed)
A web server offering similar features is available here.