Segger is a tool for segmenting 3D density maps obtained using cryo-electron microscopy (CryoEM) and fitting models to them.
- Install the latest version (an older version is already included in Chimera, but does not have all the latest features and fixes).
- Tutorials
- Report an issue or ask a question
If you use the Segment Map/Segger plugin to Chimera, citations are always appreciated:
- (2010) Quantitative analysis of cryo-EM density map segmentation by watershed and scale-space filtering, and fitting of structures by alignment to regions JSB, PubMed
- (2012) Comparison of Segger and other methods for segmentation and rigid-body docking of molecular components in cryo-EM density maps Biopolymers, Pubmed
For identifying Water & Ions (SWIM method), see Tutorials and Supplementary Materials in the following:
- (2020) Resolving individual atoms of protein complex by cryo-electron microscopy Cell Research
Newer, related papers:
- (2016) Resolution and Probabilistic Models of Components in CryoEM Maps of Mature P22 Bacteriophage Biophys. J., Pubmed
- (2019) Segmentation and Comparative Modeling in an 8.6Å Cryo-EM Map of the Singapore Grouper Iridovirus Structure, Pubmed
Related to Q-scores, evaluation of maps and map-model fit:
- (2020) Measurement of atom resolvability in cryo-EM maps with Q-scores Nature Methods
- (2020) Resolving individual atoms of protein complex by cryo-electron microscopy Cell Research
- (2021) Validation, analysis and annotation of cryo-EM structures Acta Cryst. Sect. D.
