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Scripts

First call src/suppa2_generate_events.sh to create the ioe files with events descriptions. Then, as indicated in a comment in src/suppa2_psiPerEvent.sh, group these ioe for each event type in a single one.

Separately, bring in quantifications from stringtie (see stringtie_quantifs repo). If needed, prefilter by removing outliers with R/str_q_remove_outliers.R

Run src/suppa2_psiPerEvent.sh.

Run src/suppa2_dpsi.sh, which will call src/split_events.R to group PSI by neuron type.

Analysis

Main analysis in R/dpsi.R. This script uses accessory functions defined in R/sequence_properties.R, R/sequence_conservation.R, and R/extract_event_coordinates.R.

Additional exploratory and tests scripts are stored in the branch exploratory.

Older versions

I initially thought about using Salmon for quantifications: the fact that we need to deduplicate UMIs makes it non-trivial, deleted (can be found in old commits).

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