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I require Cell Barcode / UMI calling but am unable to sort BAMs within STARsolo due to a limited system RAM.
When I set limitBAMsortRAM==<ammount of RAM on my system>, STAR throws the following error:
EXITING because of fatal ERROR: not enough memory for BAM sorting:
SOLUTION: re-run STAR with at least --limitBAMsortRAM 273246110398
Proposed solution:
Run STARsolo with
run samtools sort with my hard memory limits as follows:
I require Cell Barcode / UMI calling but am unable to sort BAMs within STARsolo due to a limited system RAM.
When I set
limitBAMsortRAM==<ammount of RAM on my system>
, STAR throws the following error:Proposed solution:
samtools sort
with my hard memory limits as follows:So how do I re-run STARsolo just to call Barcodes/UMIS on:
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