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update BMC link
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lassedochreden committed Jan 6, 2024
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Expand Up @@ -12,7 +12,7 @@ An interactive multi-channel image viewer for R.

This shiny application allows users to interactively visualize multi-channel images and segmentation masks generated by imaging mass cytometry and other highly multiplexed imaging techniques. The `cytoviewer` package is divided into image-level (Composite and Channels) and cell-level visualization (Masks). It allows users to overlay individual images with segmentation masks, integrates well with [SingleCellExperiment](https://bioconductor.org/packages/release/bioc/html/SingleCellExperiment.html) / [SpatialExperiment](https://bioconductor.org/packages/release/bioc/html/SingleCellExperiment.html) and [CytoImageList](https://www.bioconductor.org/packages/release/bioc/html/cytomapper.html) objects for metadata and image visualization and supports image downloads.

Read the **BMC Bioinformatics paper** here: [doi.org/10.1186/s12859-023-05546-z](doi.org/10.1186/s12859-023-05546-z)
Read the **BMC Bioinformatics paper** here: [doi.org/10.1186/s12859-023-05546-z](https://doi.org/10.1186/s12859-023-05546-z).

## Check status

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**(C)** *cytoviewer* supports different viewing modes. Top: The "channels" tab of image-level visualization displays individual channels. Shown are Ecad (magenta), CD8a (cyan), and CD68 (yellow) marking tumor cells, CD8+ T cells, and myeloid cells, respectively. Center: The "composite" tab of image-level visualization visualizes image composites combining multiple channels. These composite images can be overlayed with cell outlines, which can be colored by cell-specific metadata. Shown here are cell outlines colored by cell area (continous value) and cell type (categorical value; tumor cells in white). Channel color settings are as follows for all markers: Contrast: 2,5; Brightness: 1; Gamma: 1.2. Bottom: The "mask" tab can be used to visualize segmentation masks that can be colored by cell-specific metadata. Shown here are segmentation masks colored by cell area (continuous) and cell type (categorical; tumor cells in magenta). Scale bars: 150 µm.

**(D)** "Image appearance" controls can be used to add legends or titles and to change the scale bar length for image-level (top) and cell level (bottom) visualization. The cell-level mask plot depicts tumor (magenta), myeloid (yellow), and CD8+ T cells (cyan). Scale bars: 100 µm.
**(D)** "Image appearance" controls can be used to add legends or titles and to change the scale bar length for image-level (top) and cell level (bottom) visualization. The cell-level mask plot depicts tumor (magenta), myeloid (yellow), and CD8+ T cells (cyan). Scale bars: 100 µm.

**Adapted from Meyer et al., 2024**

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