diff --git a/SAMtags.tex b/SAMtags.tex index e19ec290..63761f17 100644 --- a/SAMtags.tex +++ b/SAMtags.tex @@ -9,6 +9,10 @@ \newcommand{\tagregex}[1]{{\tt #1}} \newcommand{\metavar}[1]{{\rm\emph{#1}}} +% Use as, e.g., \cigarops{MID} to produce M/I/D with the operators in \tt +\newcommand*{\cigarops}[1]{\cigaropsAux#1*} +\def\cigaropsAux#1#2*{{\tt #1}\if\relax\detokenize{#2}\relax\else/\cigaropsAux#2*\fi} + \begin{document} \input{SAMtags.ver} @@ -439,7 +443,7 @@ \subsection{Annotation and Padding} Each tag consists of \emph{start}, \emph{end}, \emph{strand}, \emph{type} and zero or more \emph{key}{\tt =}\emph{value} pairs, each separated with semicolons. \emph{Start} and \emph{end} are 1-based -positions between one and the sum of the {\tt M/I/D/P/S/=/X} +positions between one and the sum of the \cigarops{MIDPS=X} {\sf CIGAR} operators, i.e., {\sf SEQ} length plus any pads. Note any editing of the CIGAR string may require updating the {\tt PT} tag coordinates, or even invalidate them. diff --git a/SAMv1.tex b/SAMv1.tex index 9b4cb6d3..c1d76d1a 100644 --- a/SAMv1.tex +++ b/SAMv1.tex @@ -38,6 +38,10 @@ \newcommand*{\memlimited}{\textcolor{gray}{\footnotesize\it limited}} +% Use as, e.g., \cigarops{MID} to produce M/I/D with the operators in \tt +\newcommand*{\cigarops}[1]{\cigaropsAux#1*} +\def\cigaropsAux#1#2*{{\tt #1}\if\relax\detokenize{#2}\relax\else/\cigaropsAux#2*\fi} + \begin{document} \input{SAMv1.ver} @@ -423,7 +427,7 @@ \subsection{The alignment section: mandatory fields}\label{sec:alnrecord} 3 & {\sf RNAME} & String & {\tt \verb"\*"|\rnameRegexp} & Reference sequence NAME\footnotemark \\ 4 & {\sf POS} & Int & $[0,\,2^{31}-1]$ & 1-based leftmost mapping POSition \\ 5 & {\sf MAPQ} & Int & $[0,\,2^8-1]$ & MAPping Quality \\ - 6 & {\sf CIGAR} & String & {\tt \char92*|([0-9]+[MIDNSHPX=])+} & CIGAR string \\ + 6 & {\sf CIGAR} & String & {\tt \char92*|([0-9]+[MIDNSHP=X])+} & CIGAR string \\ 7 & {\sf RNEXT} & String & {\tt \verb"\*"|=|\rnameRegexp} & Reference name of the mate/next read \\ 8 & {\sf PNEXT} & Int & $[0,\,2^{31}-1]$ & Position of the mate/next read \\ 9 & {\sf TLEN} & Int & $[-2^{31}+1,\,2^{31}-1]$ & observed Template LENgth \\ @@ -554,7 +558,7 @@ \subsection{The alignment section: mandatory fields}\label{sec:alnrecord} \item For mRNA-to-genome alignment, an {\tt N} operation represents an intron. For other types of alignments, the interpretation of {\tt N} is not defined. - \item Sum of lengths of the {\tt M/I/S/=/X} operations shall equal + \item Sum of lengths of the \cigarops{MIS=X} operations shall equal the length of {\sf SEQ}. \end{itemize} \item {\sf RNEXT}: Reference sequence name of the primary alignment of the NEXT read in the @@ -638,7 +642,7 @@ \subsection{The alignment section: mandatory fields}\label{sec:alnrecord} \item {\sf SEQ}: segment SEQuence. This field can be a `*' when the sequence is not stored. If not a `*', the length of the sequence must - equal the sum of lengths of {\tt M/I/S/=/X} operations in {\sf CIGAR}. + equal the sum of lengths of \cigarops{MIS=X} operations in {\sf CIGAR}. An `=' denotes the base is identical to the reference base. No assumptions can be made on the letter cases. \item {\sf QUAL}: ASCII of base QUALity plus 33 (same as the quality @@ -725,7 +729,7 @@ \section{Recommended Practice for the SAM Format} identical to its mate. \item If all segments in a template are unmapped, their {\sf RNAME} should be set as `*' and {\sf POS} as 0. - \item If {\sf POS} plus the sum of lengths of {\tt M/=/X/D/N} + \item If {\sf POS} plus the sum of lengths of \cigarops{M=XDN} operations in {\sf CIGAR} exceeds the length specified in the {\tt LN} field of the {\tt @SQ} header line (if exists) with an SN equal to {\sf RNAME}, the alignment should be unmapped, unless the @@ -757,7 +761,7 @@ \section{Recommended Practice for the SAM Format} Mappings that cross the coordinate `join' in circular reference sequences (i.e., those whose {\tt @SQ} headers specify {\tt TP:circular}) may be represented as follows: \begin{enumerate}[label=\arabic*] \item (Preferred) -As usual {\sf POS} should be between 1 and the {\tt @SQ} header's {\tt LN} value, but {\sf POS} plus the sum of the lengths of {\tt M/=/X/D/N} {\sf CIGAR} operations may exceed {\tt LN}. +As usual {\sf POS} should be between 1 and the {\tt @SQ} header's {\tt LN} value, but {\sf POS} plus the sum of the lengths of \cigarops{M=XDN} {\sf CIGAR} operations may exceed {\tt LN}. Coordinates greater than~{\tt LN} are interpreted by subtracting {\tt LN} so that bases at $\texttt{LN}+1, \texttt{LN}+2, \texttt{LN}+3, \ldots$ are considered to be mapped at positions $1,2,3,\ldots$; thus each (1-based) position $p$ is interpreted as $((p-1)\bmod\texttt{LN})+1$.% \footnote{The impact of this representation on indexing and random access is yet to be explored by implementations.} @@ -1063,7 +1067,7 @@ \subsection{The BAM format} & \multicolumn{2}{l|}{\sf next\_pos} & 0-based leftmost pos of the next segment ($=\underline{\sf PNEXT}-1$) & {\tt int32\_t} & [-1] \\\cline{2-6} & \multicolumn{2}{l|}{\sf tlen} & Template length ($=\underline{\sf TLEN}$) & {\tt int32\_t} & [0] \\\cline{2-6} & \multicolumn{2}{l|}{\sf read\_name} & Read name, {\tt NUL}-terminated (\underline{\sf QNAME} with trailing `{\tt\verb"\0"}')\footnotemark & {\tt char[{\sf l\_read\_name}]} & \\\cline{2-6} - & \multicolumn{2}{l|}{\sf cigar} & CIGAR: {\tt {\sf op\_len}\char60\char60 4\char124{\sf op}}. `{\tt MIDNSHP\char61X}'$\to$`012345678' & {\tt uint32\_t[{\sf n\_cigar\_op}]} & \\\cline{2-6} + & \multicolumn{2}{l|}{\sf cigar} & CIGAR: {\tt {\sf op\_len}\char60\char60 4\char124{\sf op}}. `{\tt MIDNSHP=X}'$\to$`012345678' & {\tt uint32\_t[{\sf n\_cigar\_op}]} & \\\cline{2-6} & \multicolumn{2}{l|}{\sf seq} & 4-bit encoded read: `{\tt =ACMGRSVTWYHKDBN}'$\to[0,15]$. See Section~\ref{sec:seq} & {\tt uint8\_t[({\sf l\_seq}+1)/2]} & \\\cline{2-6} & \multicolumn{2}{l|}{\sf qual} & Phred-scaled base qualities. See Section~\ref{sec:seq} & {\tt char[{\sf l\_seq}]} & \\\cline{2-6} & \multicolumn{5}{c|}{\textcolor{gray}{\it List of auxiliary data (until the end of the alignment block)}} \\\cline{3-6} @@ -1513,6 +1517,7 @@ \subsection*{1.3: July 2010 to April 2011} \begin{itemize} \item Add {\tt RG PG} header field. (Nov 2010) \item Add BAM description and index sections. (Nov 2010) +\item \textbf{Add `{\tt =}' and `{\tt X}' CIGAR operations.} (July 2010) \item \textbf{Removal of FLAG letters.} (July 2010) \item The {\tt SM} header field, previously mandatory for {\tt @RG}, is now optional. (July 2010)