Q: AssertionError: No proper d can be found! Tweak --mfold?

[nanu23333]

Dear MACS2 team,

Thank you for providing this excellent tool! However, I encountered an issue when running the following command:

macs2 callpeak \ -t /DataM/hhl/task/24_11_analysis/rqh_new/06remove_duplication_call_peak/01remove_duplication/EEEE/remove_duplication/E1IP.sorted.markdup.bam \ -c /DataM/hhl/task/24_11_analysis/rqh_new/06remove_duplication_call_peak/01remove_duplication/EEEE/remove_duplication/E2ku.sorted.markdup.bam \ -n E1_IP_E2_input \ -g hs \ --outdir /DataM/hhl/task/24_11_analysis/rqh_new/06remove_duplication_call_peak/02mapping/E1_IP_E2_input/ \ -B
When executing this, I encountered the following error:

Traceback (most recent call last): File "/DataC/miniconda3/bin/macs2", line 653, in <module> main() File "/DataC/miniconda3/bin/macs2", line 51, in main run(args) File "/DataC/miniconda3/lib/python3.10/site-packages/MACS2/callpeak_cmd.py", line 168, in run peakmodel = PeakModel(treatment = treat, File "MACS2/PeakModel.pyx", line 105, in MACS2.PeakModel.PeakModel.__init__ File "MACS2/PeakModel.pyx", line 147, in MACS2.PeakModel.PeakModel.build File "MACS2/PeakModel.pyx", line 229, in MACS2.PeakModel.PeakModel.__paired_peak_model AssertionError: No proper d can be found! Tweak --mfold?
I’m wondering why this error occurs. Could it be related to my input BAM files or parameter settings? Additionally, how can I resolve this issue?

Any guidance on properly adjusting the --mfold parameter or addressing this problem would be greatly appreciated.

Thank you in advance for your support!

Best regards,
Nanu