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Add subclusters to Siletti CAS taxonomy (following IDs on ABC!) #14

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Tracked by #11
dosumis opened this issue Mar 6, 2024 · 4 comments
Open
Tracked by #11

Add subclusters to Siletti CAS taxonomy (following IDs on ABC!) #14

dosumis opened this issue Mar 6, 2024 · 4 comments

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@dosumis
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dosumis commented Mar 6, 2024

Probably needs to be done in a notebook that glues together ABC representation with cell IDs from AnnData file.

@dosumis dosumis mentioned this issue Mar 6, 2024
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@dosumis
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dosumis commented Mar 19, 2024

Plan - use cas-tools ABC2CAS to extract subclusters and parent cell set relationships to clusters. Follow naming from ABC atlas.

WHB taxonomy here:
https://allen-brain-cell-atlas.s3.us-west-2.amazonaws.com/index.html#metadata/WHB-taxonomy/20240330/

@dosumis
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dosumis commented Mar 19, 2024

Issues:

ABC conversion currently uses labelset.label (= ID)

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dosumis commented Mar 24, 2024

CAS tools is now working sufficiently well.

Proposed strategy:

  1. ABC --> CAS for Siletti
  2. Iterate over subclusters, finding which are subclusters of cluster already in CAS Silleti. Merge in all ABC subclusters with parents in to annotation dictionary (can just use dict update)
  3. Remove cellIDs
  4. Pull SubClusters from AnnData file
  5. Iterate over mapping in IDs (will require matching ID numbers to fragments of names!)

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