"prior on psi is zero!" Error During 3D Initial Reference

[Poko18]

Describe your problem
When running a 3D Initial Reference job, it starts processing but fails after some time with the error: prior on psi is zero!. Restarting the job usually helps, but we want to make our job runs more consistent. Is there a way to mitigate this error, or are there any parameters we could adjust to prevent it from happening?

Job options:

• Type of job: 3D Initial Reference

relion_refine --grad --denovo_3dref --i jobs/Extract/150/particles.star --o jobs/InitialModel/284/run --iter 150 
--K 4 --ctf --flatten_solvent --zero_mask --sym C1 --dont_combine_weights_via_disc --pool 20 --pad 2 
--particle_diameter 170 --oversampling 1 --healpix_order 1 --offset_range 6 --offset_step 4.0 
--auto_sampling --tau2_fudge 4.0 --grad_ini_resol 25 --grad_fin_resol 10 --grad_ini_subset 100 
--grad_fin_subset 400 --grad_write_iter 10 --j 12 --gpu ""

Error message:

   0/   0 sec ...........................................................~~(,_,">
   0/   0 sec ...........................................................~~(,_,">
   0/   0 sec ............................................................~~(,_,">
 Auto-refine: Estimated accuracy angles= 4.53 degrees; offsets= 2.93914 Angstroms
 Auto-refine: Angular step= 3.75 degrees; local searches= true
 Auto-refine: Offset search range= 4.99114 Angstroms; offset step= 1.5 Angstroms
 psi_angles[ipsi]= 0 prior_psi= 330
 diffpsi= 30 sigma_cutoff= 3 sigma_psi= inf
in: /tmp/relion/src/healpix_sampling.cpp, line 979
ERROR: 
prior on psi is zero!
=== Backtrace  ===
relion_refine(_ZN11RelionErrorC1ERKNSt7__cxx1112basic_stringIcSt11char_traitsIcESaIcEEES7_l+0x7d) [0x563bdc6a3efd]
relion_refine(+0x88ef6) [0x563bdc661ef6]
relion_refine(_ZN11MlOptimiser27expectationSetupCheckMemoryEi+0xc4b) [0x563bdc6c95cb]
relion_refine(_ZN11MlOptimiser11expectationEv+0x117) [0x563bdc708157]
relion_refine(_ZN11MlOptimiser7iterateEv+0xdb) [0x563bdc71756b]
relion_refine(main+0x6f) [0x563bdc69207f]
/usr/lib/x86_64-linux-gnu/libc.so.6(__libc_start_main+0xf3) [0x7fb073b41083]
relion_refine(_start+0x2e) [0x563bdc695cee]
==================
ERROR: 
prior on psi is zero!

---

Environment:

• RELION version: 5.0-beta-2-commit-312258
• Memory: 128Gb
• GPU: A10G

Dataset:

• Box size: 288
• Pixel size: 0.86 apix (2.06 extracted)
• Number of particles: 1000000